Transformation of canola by chit33 gene towards improving resistance to Sclerotinia sclerotiorum
An endochitinase gene (chit33-cDNA) from the biocontrol fungus Trichoderma atroviride was overexpressed under the CaMV35S constitutive promoter in canola (R line Hyola 308).Transformation of cotyledonary petioles was achieved 3 NATURAL BROWN via Agrobacterium tumefaciens.The insertion of the transgene was verified by PCR and Southern blotting.The t